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首頁(yè) > 技術(shù)文章 > BioGems重組人轉(zhuǎn)鐵蛋白的性質(zhì)

BioGems重組人轉(zhuǎn)鐵蛋白的性質(zhì)

更新時(shí)間:2018-04-18瀏覽:1964次

BioGems重組人轉(zhuǎn)鐵蛋白是在無(wú)動(dòng)物源的GMP車間生產(chǎn)的,活性相當(dāng)于人血清鐵飽和的轉(zhuǎn)鐵蛋白(holo transferrin),純度≥ 95% 。適用于細(xì)胞培養(yǎng)。

 

BioGems重組人轉(zhuǎn)鐵蛋白參數(shù)

別名 Serotransferrin(血清轉(zhuǎn)鐵蛋白), TF(轉(zhuǎn)鐵蛋白), TFQTL1, PRO2086, PRO1557

 

表達(dá)系統(tǒng) Oryza Sativa 水稻

 

氨基酸序列 VPDKTVRWC AVSEHEATK CQSFRDHMK SVIPSDGPS VACVKKASY LDCIRAIAA NEADAVTLD AGLVYDAYL APNNLKPVV AEFYGSKED PQTFYYAVA VVKKDSGFQ MNQLRGKKS CHTGLGRSA GWNIPIGLL YCDLPEPRK PLEKAVANF FSGSCAPCA DGTDFPQLC QLCPGCGCS TLNQYFGYS GAFKCLKDG AGDVAFVKH STIFENLAN KADRDQYEL LCLDNTRKP VDEYKDCHL AQVPSHTVV ARSMGGKED LIWELLNQA QEHFGKDKS KEFQLFSSP HGKDLLFKD SAHGFLKVP PRMDAKMYL GYEYVTAIR NLREGTCPE APTDECKPV KWCALSHHE RLKCDEWSV NSVGKIECV SAETTEDCI AKIMNGEAD AMSLDGGFV YIAGKCGLV PVLAENYNK SDNCEDTPE AGYFAIAVV KKSASDLTW DNLKGKKSC HTAVGRTAG WNIPMGLLY NKINHCRFD EFFSEGCAP GSKKDSSLC KLCMGSGLN LCEPNNKEG YYGYTGAFR CLVEKGDVA FVKHQTVPQ NTGGKNPDP WAKNLNEKD YELLCLDGT RKPVEEYAN CHLARAPNH AVVTRKDKE ACVHKILRQHLFGSNV TDCSGNFCL FRSETKDLL FRDDTVCLA KLHDRNTYE KYLGEEYVK AVGNLRKCS TSSLLEACT FRRP

 

質(zhì)檢 通過(guò)N端和質(zhì)譜分析進(jìn)行驗(yàn)證(適用時(shí))。Verified by N-terminal and Mass Spectrometry analyses(when applicable).

 

純度 ≥ 95% by SDS-PAGE.

 

內(nèi)毒素水平 Endotoxin level is < 1 EU/mg.

 

生物活性 在無(wú)血清添加培養(yǎng)基中通過(guò)與天然人轉(zhuǎn)鐵蛋白在雜交瘤細(xì)胞的抗體生產(chǎn)中比較來(lái)證實(shí)生物學(xué)活性。Biological activity verified by comparison against native human transferrin in the antibody production of hybridoma cells in serum-free supplemented media.

 

處理 0.2μm滅菌過(guò)濾。從磷酸鈉和氯化鈉溶液(pH7.0)凍干。