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品牌 | illumina/美國因美納 | 供貨周期 | 一個(gè)月 |
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Illumina Miseq測(cè)序儀測(cè)序必需試劑盒,包括簇生成、雙端測(cè)序和邊合成邊測(cè)序試劑(SBS)。
產(chǎn)品英文名稱:MiSeq Kit
產(chǎn)品中文名稱:MiSeq Kit試劑盒
用途:小型全基因組測(cè)序(微生物、病毒),靶向基因測(cè)序(擴(kuò)增子、基因panel),16S宏基因組測(cè)序,靶向基因表達(dá)譜分析,miRNA和Small RNA分析,長片段擴(kuò)增子測(cè)序,DNA-蛋白質(zhì)相互作用分析
貨號(hào):
MS-102-2001/MS-102-2002/MS-102-2003/MS-102-3001/MS-102-3003/MS-103-1001/MS-103-1002/MS-103-1003
要在 MiSeq 上執(zhí)行運(yùn)行,需要一個(gè)一次性 MiSeq 試劑盒。
該試劑盒有各種不同的類型和規(guī)格。每種類型的 MiSeq 試劑盒都包含一個(gè)試劑盒的流動(dòng)槽類型和執(zhí)行一次運(yùn)行所需的全部試劑。試劑盒中提供的流動(dòng)槽、PR2 瓶和試劑盒均使用射頻識(shí)別(radio-frequency identification,簡(jiǎn)稱 RFID)進(jìn)行精確的耗材跟蹤并確保兼容性。始終使用與您的流動(dòng)槽類型相匹配的試劑盒。如果試劑盒不兼容,運(yùn)行設(shè)置期間會(huì)顯示一條消息,提示您裝入兼容的試劑盒。
流動(dòng)槽:flow cell
MiSeq Kit特點(diǎn):
改良化學(xué)過程后,循環(huán)時(shí)間更快、讀長(reads)更長、輸出更多
雙表面成像實(shí)現(xiàn)的讀長數(shù)是v1試劑盒單表面成像的兩倍
使用500次循環(huán)的試劑盒可擴(kuò)展讀長長度
選擇您應(yīng)用的循環(huán)次數(shù)(50、300或500)
借助MiSeq試劑盒v3,研究人員可:
使單次運(yùn)行的輸出翻倍
使用600次循環(huán)試劑盒,使reads長度zui長延伸至2 x 300堿基對(duì)
將reads數(shù)量增至2500萬個(gè),以開創(chuàng)新的應(yīng)用
The MiSeq Reagent Kit is a single-use reagent kit required to perform a sequencing run. It is available in different types and sizes. Each type of MiSeq Reagent Kit includes a kit-specific flow cell type and all reagents required for performing a run.The flow cell, PR2 bottle, and reagent cartridge provided in the kit use radio-frequency identification (RFID) for accurate consumable tracking and compatibility.Always use the reagent cartridge associated with your flow cell type. If the reagent cartridge is not compatible, a message appears during run setup that prompts you to load a compatible reagent cartridge.
Get faster cycle times, longer reads and more output from improved chemistry
Dual surface imaging enables twice the number of reads compared to single surface imaging with the v1 kit
Extend your read lengths with the 500-cycle kit
Choose the perfect number of cycles for your application (50, 300, or 500)
MiSeq Reagent Kit v3 allows researchers to:
Double the output of a single run
Extend read lengths up to 2 x 300 bp with the 600-cycle kit
Increase the number of reads to 25 million to unlock new applications
MiSeq Kit應(yīng)用
小型全基因組測(cè)序(微生物、病毒),靶向基因測(cè)序(擴(kuò)增子、基因panel),16S宏基因組測(cè)序,靶向基因表達(dá)譜分析,miRNA和Small RNA分析,長片段擴(kuò)增子測(cè)序,DNA-蛋白質(zhì)相互作用分析
實(shí)例:
上游試劑 癌癥panel:TruSeq Amplicon - Cancer Panel
zui高通量 | 8.5 Gb (500-cycle MiSeq Reagent Kit v2), 5.1 Gb (300-cycle MiSeq Reagent Kit v2), 1.2 Gb (300-cycle MiSeq Reagent Micro Kit v2), 0.850 Gb (50-cycle MiSeq Reagent Kit v2), 0.5 Gb (500-cycle MiSeq Reagent Nano Kit v2), 0.3 Gb (300-cycle MiSeq Reagent Nano Kit v2),15 Gb (600-cycle MiSeq Reagent Kit v3), 3.8 Gb (150-cycle MiSeq Reagent Kit v3) |
zui高讀長 | Up to 15 million(v2),Up to 25 Million(v3) |
試劑類型 | Cluster Generation, Paired-End Sequencing, Sequencing by Synthesis |
測(cè)序儀 | MiSeq, MiSeq FGx in Research Mode, MiSeqDx in Research Mode |
組份 | 數(shù)量 | 描述 | 保存 |
Reagent Cartridge | 1 | Single-use pre-filled cartridge | -25°C to -15°C |
HT1 | 1 | 5 ml tube, Hybridization Buffer | -25°C to -15°C |
Flow Cell | 1 | Single-use flow cell | 2°C to 8°C |
PR2 | 1 | 500 ml bottle, Incorporation Buffer | 2°C to 8°C |
自備試劑:
備用的 1.0 N NaOH,分子生物學(xué)級(jí)
酒精擦拭紙,70%甘油或乙醇,70%
一次性無粉手套
低塵實(shí)驗(yàn)室棉巾紙
微量離心管
擦鏡紙,4 x 6 英寸
MiSeq試管,Illumina
NaOCl,5%
Tween 20
實(shí)驗(yàn)室級(jí)用水
品牌 | 貨號(hào) | 名稱 | 規(guī)格 |
Illumina | MS-102-2001 | MiSeq Reagent Kit v2 | 50 cycles |
Illumina | MS-102-2002 | MiSeq Reagent Kit v2 | 300 cycles |
Illumina | MS-102-2003 | MiSeq Reagent Kit v2 | 500 cycles |
Illumina | MS-102-3001 | MiSeq Reagent Kit v3 | 150 cycles |
Illumina | MS-102-3003 | MiSeq Reagent Kit v3 | 600 cycles |
Illumina | MS-103-1001 | MiSeq Reagent Nano Kit v2 | 300 cycles |
Illumina | MS-103-1002 | MiSeq Reagent Micro Kit v2 | 300 cycles |
Illumina | MS-103-1003 | MiSeq Reagent Nano Kit v2 | 500 cycles |
注:micro 和 nano的區(qū)別在于測(cè)序通量。
Illumina- MiSeq Kit及更多測(cè)序產(chǎn)品詳詢
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