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品牌 | GIBCO/美國 | 貨號 | 11131D |
---|---|---|---|
規(guī)格 | 2 mL | 供貨周期 | 現(xiàn)貨 |
主要用途 | T 細(xì)胞活化和擴增 |
Dynabeads™ Human T-Activator CD3/CD28 免疫磁珠通過偶聯(lián)CD3/CD28單抗,活化擴增人T細(xì)胞。
產(chǎn)品英文名 Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation
產(chǎn)品中文名 Dynabeads™ Human T-Activator CD3/CD28 免疫磁珠
產(chǎn)品介紹 Dynabeads® Human T-Activator CD3/CD28 用于活化和擴增人 T 細(xì)胞和 T 細(xì)胞克隆,以作研究之用。Dynabeads® CD3/CD28 T Cell Expander 提供了活化和擴增 T 細(xì)胞的簡單方法,無需抗原呈遞細(xì)胞或抗原。 Dynabeads® Human T-Activator CD3/CD28 是均勻的 4.5 µm 超順磁珠,與抗 CD3 和抗 CD28 單克隆抗體的優(yōu)化混合物偶聯(lián)。 通過使抗 CD3 和抗 CD28 抗體與 Dynabeads® 結(jié)合,這種微珠可以提供 T 細(xì)胞活化和擴增所需的原始和共刺激信號。
Dynabeads免疫磁珠
品牌 | 貨號 | 產(chǎn)品描述 | 包裝 | 價格 |
Thermo | 11131D | Dynabeads™ Human T-Activator CD3/CD28 磁珠 | 2 mL | 8385元 |
Thermo | 11132D | Dynabeads™ Human T-Activator CD3/CD28 磁珠 | 5 x 2 mL | 26006元 |
Thermo | 11161D | Dynabeads™ Human T-Activator CD3/CD28 磁珠 | 0.4 mL | 1575 元 |
歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司:紅榮微再(上海)生物工程技術(shù)有限公司 :1500 1904 520。紅榮微再-客服: 經(jīng)銷商專員
起始樣品
Dynabeads免疫磁珠適用來自全血或血沉棕黃層的 MNC/PBMC,或者包含 CD3+ T 細(xì)胞、CD4+ 或 CD8+ T 細(xì)胞、調(diào)節(jié)性 T 細(xì)胞或 T 細(xì)胞克隆的免疫系統(tǒng)T 細(xì)胞亞群。
擴增效果
人T細(xì)胞擴增:12天擴增3000倍
規(guī)格
Cell Type: T Cells (Whole Population)
Clonality: Monoclonal
Final Product: Cells
Product Line: DYNAL®, Dynabeads®
Reactivity: Human
Sample Type (Specific): Cell Cultures, PBMC
Target Species: Human
Shipping Condition: Room Temperature
細(xì)胞類型:免疫T細(xì)胞
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產(chǎn)物:細(xì)胞
產(chǎn)品線:DYNAL®, Dynabeads®
反應(yīng)性:人
樣本類型:細(xì)胞培養(yǎng)物,PBMC(外周血單個核細(xì)胞)
靶種屬:人
運輸條件:室溫
內(nèi)容及儲存
Dynabeads® Human T-Activator CD3/CD28 are supplied as a suspension containing 4 × 107 Dynabeads®/ml in phosphate buffered saline (PBS), pH 7.4, with 0.1% human serum albumin (HSA). Store at: 2-8°C.
Dynabeads免疫磁珠是一種懸液,4 × 107 Dynabeads®/ml的磁珠溶于PBS,pH 7.4,另含0.1%人白蛋白。
2-8°C儲存。
Dynabeads免疫磁珠特點
Dynabeads® Human T-Activator CD3/CD28 are for the activation and expansion of human T cells. Advantages of Dynabeads® Human T-Activator CD3/CD28:
• Activation of T cells without the need for feeder cells
T細(xì)胞活化無需滋養(yǎng)層細(xì)胞
• Activated cells that retain in vivo-like function
活化細(xì)胞功能同體內(nèi)培養(yǎng)
• Maximum reproducibility without contamination by soluble antibodies or mitogens
zui高可重復(fù)性,沒有抗體溶解和絲裂原污染。
• From 100- to 1,000-fold T cell expansion
T細(xì)胞擴增100-1000倍
特別應(yīng)用
CAR-T細(xì)胞活化擴增:
CAR-T免疫療法
繼諾華和Kite公司兩款CAR-T細(xì)胞治療被FDA批準(zhǔn),2017年被媒體評為CAR-T療法的元年。
CAR T細(xì)胞免疫療法中,因為純化自體抗原呈遞細(xì)胞(APCs)用于T細(xì)胞活化需要好幾個額外步驟,增加獲得CAR T細(xì)胞產(chǎn)品強度和難度。因此研究者開發(fā)了另外一種更標(biāo)準(zhǔn)化、更具效率的T細(xì)胞活化方法,比如使用抗CD3/抗CD28單克隆抗體(anti-CD3/anti-CD28 monoclonal antibodies)。
而Dynabeads® Human T-Activator CD3/CD28 這種微珠可以提供 T 細(xì)胞活化和擴增所需的原始和共刺激信號,通過使抗 CD3和抗 CD28 抗體與 Dynabeads® 結(jié)合,更加速擴增。
推薦PeproTech抗CD3/抗CD28單克隆抗體產(chǎn)品
T細(xì)胞活化抗人CD3單克隆抗體(Functional,clone: OKT3)可以與TCR復(fù)合物結(jié)合,激活T細(xì)胞。SAFIRE(Sodium Azide Free Immensely Reduced Endotoxins) 類產(chǎn)品是BioGems品牌下的功能性抗體產(chǎn)品。
品牌 | 產(chǎn)品名稱 | 貨號 | 規(guī)格 | 單價 | * |
PeproTech/BioGems | Anti-Human CD3 SAFIRE Purified | 05121-25-500 | 500 μg | ¥4,130 | ¥1,652 |
PeproTech/BioGems | Anti-Human CD3 SAFIRE Purified | 05121-25-100 | 100 μg | ¥2,240 |
|
PeproTech/BioGems | Anti-Human CD28 SAFIRE Purified | 10311-25-500 | 500 μg | ¥3,640 | ¥1,456 |
PeproTech/BioGems | Anti-Human CD28 SAFIRE Purified | 10311-25-100 | 100 μg | ¥1,820 |
|
歡迎您致電 華雅再生醫(yī)學(xué)旗艦公司:紅榮微再(上海)生物工程技術(shù)有限公司 :1500 1904 520。紅榮微再-客服: 經(jīng)銷商專員
About Dynabeads® Human T-Activator CD3/CD28
Dynabeads® Human T-Activator CD3/CD28 offer a simple method for activation and expansion of T cells that does not require feeder cells (antigen-presenting cells) or antigen. The uniform 4.5 µm diameter, inert, superparamagnetic beads are similar in size to antigen-presenting cells, and are covalently coupled to anti-CD3 and anti-CD28 antibodies. These two antibodies provide primary and co-stimulatory signals, optimized for efficient T cell activation and expansion. Expansion of the T cell population can be stimulated using recombinant IL-2, and after activation or expansion, the magnetic beads can be easily removed using a DynaMag™ magnet. For expansion of antigen-specific T cells from T cell clones or T cell lines, we recommend using Dynabeads® Human T-Activator CD3/CD28/CD137.
Starting samples
Start with mononuclear cells (MNCs)/peripheral blood mononuclear cells (PBMCs) from either whole blood or buffy coat, or T cell subsets including CD3+ T cells, CD4+ or CD8+ T cells, or regulatory T cells.
Downstream applications
The activated T cells can be analyzed shortly after activation (for transfection/transduction or to study TCR signaling, proteomics, gene expression, etc.). T cells can be left in culture to differentiate into T helper cell subsets or expansion of polyclonal/antigen-specific T cells.
For Research Use Only. Not for use in diagnostic procedures.
引用及參考文獻
Activated human CD4+ T cells express transporters for both cysteine and cystine.
Levring TB, Hansen AK, Nielsen BL, Kongsbak M, von Essen MR, Woetmann A, Odum N, Bonefeld CM, Geisler C, Sci Rep (2012) 2:266-266
Dasatinib, a small molecule protein tyrosine kinase inhibitor, inhibits T cell activation and proliferation.
Schade AE, Schieven GL, Townsend R, Jankowska AM, Susulic V, Zhang R, Szpurka H, Maciejewski JP, Blood 2007 0(0):-
Loss of Siglec expression on T lymphocytes during human evolution.
Nguyen DH, Hurtado-Ziola N, Gagneux P, Varki A
Proc Natl Acad Sci U S A (2006) 103:7765-7770